Journal: BioMedicine

Article Title: Decyl caffeate inhibits the proliferation of human triple negative breast cancer cells

doi: 10.37796/2211-8039.1695

Figure Lengend Snippet: CA derivatives suppress cell proliferation and clonogenic growth in TNBC cell lines. (A) Structural representations of CA, EC, and DC. (B, C) MDA-MB-468, MDAMB-157, and MDA-MB-231 cells were treated with 0, 10, 20, or 40 μM of CA derivatives for 24 h (B) and 48 h (C). Cell viability was assessed using the MTT assay. (D) Long-term proliferative capacity was evaluated through colony formation assays. Distinct lowercase, uppercase, or double uppercase letters denote statistically significant differences (P < 0.05) within the CA, EC, and DC treatment sets, respectively. (E) Synergistic effects of EC or DC (0–40 μM) with 10 μM cisplatin on MDA-MB468 cell viability were measured after 24 h. P < 0.05: single asterisk (*) denotes significance versus the untreated control; double asterisk (**) versus cisplatin alone.

Article Snippet: Triple-negative breast cancer (TNBC) cell lines— MDA-MB-468 (ATCC® HTB-132), MDA-MB-157 (ATCC® HTB-24), and MDA-MB-231 (ATCC® HTB26)—were acquired from the American Type Culture Collection (ATCC, Walkersville, MD, USA) and validated for authenticity.

Techniques: MTT Assay, Control

Journal: BioMedicine

Article Title: Decyl caffeate inhibits the proliferation of human triple negative breast cancer cells

doi: 10.37796/2211-8039.1695

Figure Lengend Snippet: DC promotes apoptosis in TNBC Cells via caspase-dependent mechanisms. (A) Flow cytometry was used to assess apoptosis via annexin V-FITC and PI staining after 24-h exposure to CA or DC. Quantification of apoptotic cells (early and late stages); data represent means ± standard deviation (SD) from three independent replicates. Representative micrographs depicting the morphological alterations in MDA-MB-468 cells following treatment with 40 μM DC were presented. (B) Western blotting was performed on cytoplasmic and nuclear extracts to assess Bax, cleaved caspase-3, cleaved-PARP, and pro-PARP expression. Actin and lamin A served as cytoplasmic and nuclear controls, respectively. A single asterisk (*) denotes significant differences versus control. (C) Cells were treated with DC (0–40 μM) in the presence or absence of pancaspase inhibitor Z-VAD-FMK (50 μM), followed by viability assessment. A single asterisk indicates significance compared to the control subgroup.

Article Snippet: Triple-negative breast cancer (TNBC) cell lines— MDA-MB-468 (ATCC® HTB-132), MDA-MB-157 (ATCC® HTB-24), and MDA-MB-231 (ATCC® HTB26)—were acquired from the American Type Culture Collection (ATCC, Walkersville, MD, USA) and validated for authenticity.

Techniques: Flow Cytometry, Staining, Standard Deviation, Western Blot, Expressing, Control

Journal: BioMedicine

Article Title: Decyl caffeate inhibits the proliferation of human triple negative breast cancer cells

doi: 10.37796/2211-8039.1695

Figure Lengend Snippet: DC Disrupts Akt/mTOR and MAPK/ERK Pathways in TNBC Cells. Cells were treated with DC (0–40 μM) for 24 h in FBS-supplemented L-15 medium. Western blotting assay of phospho-Akt (Thr308 and Ser473), phospho-ERK1/2 (Thr202/Tyr204), and phospho-mTOR (Ser2448) was performed. Total levels of Akt and β-actin were included as controls. A single asterisk (*) denotes statistically significant differences from untreated controls (P < 0.05).

Article Snippet: Triple-negative breast cancer (TNBC) cell lines— MDA-MB-468 (ATCC® HTB-132), MDA-MB-157 (ATCC® HTB-24), and MDA-MB-231 (ATCC® HTB26)—were acquired from the American Type Culture Collection (ATCC, Walkersville, MD, USA) and validated for authenticity.

Techniques: Western Blot

Journal: BioMedicine

Article Title: Decyl caffeate inhibits the proliferation of human triple negative breast cancer cells

doi: 10.37796/2211-8039.1695

Figure Lengend Snippet: DC Suppresses Tumor Growth in a TNBC Xenograft Model. NOD SCID mice bearing MDA-MB-468 xenografts were treated with DC (2 mg/kg/day) or vehicle for six weeks. (A) Tumor volume was recorded weekly. Distinct letters at each time point indicate significant differences (P < 0.05). (B) Tumor weights at study endpoint; a single asterisk (*) indicates P < 0.05. Body weight (C) and food consumption (D) were monitored following DC administration in xenograft-bearing mice. Data are expressed as mean ± SD, and no statistically significant differences were observed between treatment and control groups. Histological examination of tumor (E) and liver tissues (F) (H&E stained) at 100× and 200× magnification. Blue and red indicate hematoxylin-stained nuclei and eosin-stained cytoplasm, respectively.

Article Snippet: Triple-negative breast cancer (TNBC) cell lines— MDA-MB-468 (ATCC® HTB-132), MDA-MB-157 (ATCC® HTB-24), and MDA-MB-231 (ATCC® HTB26)—were acquired from the American Type Culture Collection (ATCC, Walkersville, MD, USA) and validated for authenticity.

Techniques: Control, Staining

Journal: BioMedicine

Article Title: Decyl caffeate inhibits the proliferation of human triple negative breast cancer cells

doi: 10.37796/2211-8039.1695

Figure Lengend Snippet: Schematic overview of DC-mediated inhibition of PI3K/Akt/mTOR and MAPK/ERK signaling in TNBC cells. The proposed mechanism illustrates how DC interferes with critical survival and cell cycle pathways, leading to growth inhibition and apoptosis in TNBC. : induction; : suppression.

Article Snippet: Triple-negative breast cancer (TNBC) cell lines— MDA-MB-468 (ATCC® HTB-132), MDA-MB-157 (ATCC® HTB-24), and MDA-MB-231 (ATCC® HTB26)—were acquired from the American Type Culture Collection (ATCC, Walkersville, MD, USA) and validated for authenticity.

Techniques: Inhibition